Immunofluorescence staining of CCL2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CCL2 polyclonal antibody (dilution ratio 1:300) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Golgi apparatus.
CCL2 / MCP1 Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C818150-100
Catalog Number: LS-C818150
For bulk orders or custom formulations:
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- Description
- MCP1 antibody LS-C818150 is an unconjugated rabbit polyclonal antibody to human MCP1 (CCL2). Validated for ELISA, ICC, IF and IHC.
- Application
- ELISA, ICC, IF, IHC-P
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- C-C motif chemokine 2 | Chemokine (C-C motif) ligand 2 | GDCF-2 | HC11 | HSMCR30 | MCP1 | SCYA2 | Small-inducible cytokine A2 | Monocyte secretory protein JE | CCL2 | MCAF | MCP-1 | Monocyte chemotactic protein 1 | SMC-CF
- Recommended Dilution: ELISA
- 1:5000 - 1:10000
- Recommended Dilution: IHC-P
- 1:500 - 1:2000
- UniProt Number
- P13500
- NCBI GenBank
- NM_002982 | NP_002973.1
- SwissProt
- P13500
Target
- Target
- Human CCL2 / MCP1
- Antigen Species
- Human
- Epitope
- Human MCP-1 / CCL2
- Gene Name
- CCL2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Human MCP-1 / CCL2 Protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS