CBX3 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg A549 Whole Cell Lysate. 2 uL anti-CBX3 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-CBX3 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa.
CBX3 / HP1 Gamma Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C814750-100
Catalog Number: LS-C814750
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- Description
- HP1 Gamma antibody LS-C814750 is an unconjugated rabbit polyclonal antibody to human HP1 Gamma (CBX3). Validated for ICC, IF, IP and WB.
- Application
- ICC, IF, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CBX3 | Chromobox homolog 3 | HP1Hs-gamma | Heterochromatin-like protein 1 | Modifier 2 protein | HP1-GAMMA | Chromobox protein homolog 3 | HECH | HP1 gamma | HP1 gamma homolog
- Recommended Dilution: IF/ICC
- 1:300 - 1:10000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q13185
- NCBI GenBank
- NM_016587 | NP_057671.2
- SwissProt
- Q13185
Target
- Target
- Human CBX3 / HP1 Gamma
- Antigen Species
- Human
- Epitope
- Human HP1 Gamma/CBX3
- Gene Name
- CBX3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to the center region of the Human CBX3
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS