Western blot of human RUNX2in Saos-2 cell lysate in the 1) absence and 2) presence of immunizing peptide using antibody at2 ug/ml.
CBFA1 / RUNX2 Antibody (aa250-300)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Bovine, Chicken, Dog, Hamster, Horse, Human, Mammal (Other), Mouse, Non-Human Primate, Pig |
| Format: | Liquid |
$394.00each
Catalog Number: LS-C47340-100
Catalog Number: LS-C47340
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- Description
- RUNX2 antibody LS-C47340 is an unconjugated rabbit polyclonal antibody to RUNX2 (CBFA1) (aa250-300) from human. It is reactive with human, mouse, bat and other species. Validated for WB.
- Application
- IHC, WB
- Reactivity
- Bov, Can, Ck, Ham, Hr, Hu, Mml, Ms, NHP, Por
- Predicted Reactivity
- Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- AML3 | CCD | CCD1 | OSF2 | PEBP2A1 | PEBP2A2 | PEA2-alpha A | PEBP2-alpha A | OSF-2 | PEBP2A | PEBP2aA1 | RUNX2 | CBF-alpha-1 | CBFA1 | Oncogene AML-3 | PEA2aA | PEBP2aA
- Recommended Dilution: WB
- 1 - 3 µg/ml
- UniProt Number
- Q13950
- NCBI GenBank
- NM_004348 | NP_004339.3
- SwissProt
- Q13950
Target
- Target
- Human CBFA1 / RUNX2
- Antigen Species
- Human
- Epitope
- A portion of amino acids 250-300 of human RUNX2 RUNX2 and RUNX1 share an approximate 66% homology in peptide sequence used as immunogen.
- Gene Name
- RUNX2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A synthetic peptide (NPQGQSQITDPRQAQS) corresponding to amino acids 264-279 of human RUNX2 was used as immunogen for this antibody. RUNX2 and RUNX1 share an approximate 66% homology in peptide sequence used as immunogen. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Pig, Opossum, Turkey, Sparrow, Chicken (100%); Rat, Platypus, Lizard (94%); Xenopus, Medaka, Pufferfish, Zebrafish, Stickleback (88%).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.05% Sodium Azide, 0.2% Gelatin
- Concentration
- 0.5 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS