Western Blot of rabbit anti-Catalase antibody. Lane R: Reduced samples of purified Catalase. Lane NR: Non-reduced samples of purified Catalase. Load: ~1ug of protein per lane. Primary antibody: Catalase antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight 488 rabbit secondary antibody at 1:10,000 for 1.5 hrs at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: 59.9 kDa, 55 kDa for catalase. Other band(s): none.
CAT / Catalase Antibody (Liquid, Biotin)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Bovine |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745131-25
Catalog Number: LS-C745131
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- Description
- Catalase antibody LS-C745131 is a biotin-conjugated rabbit polyclonal antibody to bovine Catalase (CAT). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Bov
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Catalase | CAT
- Recommended Dilution: ELISA
- 1:10000 - 1:40000
- Recommended Dilution: WB
- 1:500 - 1:2500
- UniProt Number
- P04040
- NCBI GenBank
- NM_001752, NP_001743.1
- SwissProt
- P04040
Target
- Target
- Bovine CAT / Catalase
- Antigen Species
- Bov
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Catalase [Bovine Liver]. Cross reactivity against Catalase from other sources may occur but has not been specifically determined.
- Gene Name
- CAT
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Hu
Immunogen
- Immunogen
- Catalase [Bovine Liver].
Format
- Conjugate/Label
- Biotin
- Format
- Liquid
- Formulation
- 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- IEX
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl