CASP9 was immunoprecipitated using: Lane A: 0.5 mg NIH-3T3 Whole Cell Lysate. 4 uL anti-CASP9 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-CASP9 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 46 kDa.
CASP9 / Caspase 9 Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C815248-100
Catalog Number: LS-C815248
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- Description
- Caspase 9 antibody LS-C815248 is an unconjugated rabbit polyclonal antibody to human Caspase 9 (CASP9). Validated for ICC, IF, IP and WB.
- Application
- ICC, IF, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- APAF3 | CASP9 | CASPASE-9c | ICE-LAP6 | MCH6 | ICE-like apoptotic protease 6 | APAF-3 | Apoptotic protease Mch-6 | CASP-9 | Caspase 9 | Caspase-9 | PPP1R56
- Recommended Dilution: IF/ICC
- 1:300 - 1:10000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P55211
- NCBI GenBank
- NM_001229 | NP_001220.2
- SwissProt
- P55211
Target
- Target
- Human CASP9 / Caspase 9
- Antigen Species
- Human
- Epitope
- Human Caspase-9
- Gene Name
- CASP9
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to the C-Terminus of Human Caspase-9
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS