LSBio
CASP3 / Caspase 3 Antibody (N-Terminus)

CASP3 / Caspase 3 Antibody (N-Terminus)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Western Blot
Reactivity:Bovine, Chicken, Dog, Fungus / Yeast / Mold, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat, Sheep
Format:Liquid
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$576.00each
Catalog Number: LS-C12494-50

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Overview

Description
Caspase 3 antibody LS-C12494 is an unconjugated rabbit polyclonal antibody to Caspase 3 (CASP3) (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for WB.
Application
WB
Reactivity
Bov, Can, Ck, Fung, GP, Hu, Ms, Por, Rb, Rt, Sh
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Apopain | CASP-3 | CASP3 | Caspase-3 | CPP32B | CPP32 | Cysteine protease CPP32 | SCA-1 | SREBP cleavage activity 1 | Yama | Procaspase3 | Caspase 3 | CPP-32 | PARP cleavage protease | Protein Yama
UniProt Number
P42574
SwissProt
P42574

Target

Target
Human CASP3 / Caspase 3
Antigen Species
Human
Epitope
Recognizes human caspase-3. In apoptotic cells, this antibody also detects 20kD and 18kD proteins which correspond to the processed forms of caspase-3. Following the induction of apoptosis and the appearance of these smaller bands, a concurrent reduction in the amount of the procaspase enzyme is noted on immunoblot analysis. A weak ~30kD band may be detected in some samples. This band is not caspase-3 as it is detected in MCF7 cell lysates which lack the caspase-3 protein. Species cross-reactivity: mouse, rat, bovine, canine, chicken, guinea pig, hamster, monkey, porcine, rabbit and yeast.
Gene Name
CASP3

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Recombinant full length human caspase-3 protein with an N-Terminus (His) 6 tag.
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.2, 0.09% Sodium Azide, 50% Glycerol
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.