Detection of Human and Mouse CARF by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (H; 50 ug), Jurkat (J; 50 ug), and NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CARF antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
CARF / ALS2CR8 Antibody (aa1-50)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C290514-10
Catalog Number: LS-C290514
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- Description
- ALS2CR8 antibody LS-C290514 is an unconjugated rabbit polyclonal antibody to ALS2CR8 (CARF) (aa1-50) from human. It is reactive with human and mouse. Validated for IP and WB.
- Application
- IP, WB
- Reactivity
- Hu, Ms
- Predicted Reactivity
- Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CARF | ALS2CR8 | NYD-SP24 | Calcium-response factor
- Recommended Dilution: WB
- 1:1000 - 1:5000
- UniProt Number
- Q8N187
- NCBI GenBank
- NM_024744 | NP_079020.13
- SwissProt
- Q8N187
Target
- Target
- Human CARF / ALS2CR8
- Antigen Species
- Human
- Epitope
- Region between residue 1 and 50 of human Collaborator of ARF using the numbering given in entry NP_060102.1 (GeneID 55602).
- Gene Name
- CARF
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 55602 corresponding to amino acids between 1 and 50 of UniprotID: Q9NXV6
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- Tris-citrate