Western blot of TUCAN in five matched pairs of normal colonic mucosa (N) and colon carcinoma (T) using Polyclonal Antibody to TUCAN/CARD8 at 1:2000. Specimens were normalized for total protein content. Each set of N and T is tissue lysate from the same patient.
CARD8 / Cardinal / TUCAN Antibody (aa125-146)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$394.00each
Catalog Number: LS-C148306-50
Catalog Number: LS-C148306
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- Description
- TUCAN antibody LS-C148306 is an unconjugated rabbit polyclonal antibody to human TUCAN (CARD8 / Cardinal) (aa125-146). Validated for IHC, IP and WB.
- Application
- IHC, IHC-F, IHC-P, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Apoptotic protein NDPP1 | DAKAR | KIAA0955 | NDPP | TUCAN | CARD8 | CARDINAL | DACAR | NDPP1
- Recommended Dilution: IHC-P
- 1:1000 - 1:5000
- Recommended Dilution: IP
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:1000 - 1:2000
- UniProt Number
- Q9Y2G2
- NCBI GenBank
- NM_014959 | NP_055774.2
- SwissProt
- Q9Y2G2
Target
- Target
- Human CARD8 / Cardinal / TUCAN
- Antigen Species
- Human
- Epitope
- The gene encoding TUCAN produces, by alternative splicing, multiple transcripts, with introns, putatively encoding numerous different protein isoforms. Thus the molecular weight observed by SDS-PAGE can vary depending on the TUCAN form expressed.
- Gene Name
- CARD8
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- A synthetic peptide corresponding to amino acids 125-146 (LVGGPLFDVTAEPEEAVAEIHL) of human TUCAN (CARD8), GenBank no. gi|14424229|sp|Q9Y2G2|CARD8_HUMAN. Percent identity by BLAST analysis: Human, Gorilla (100%); Orangutan, Gibbon (95%); Monkey, Marmoset (86%).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Antiserum, 0.05% sodium azide
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Unpurified (Serum / Ascites / Supernatant)
Storage & Handling
- Storage Conditions
- Store at -20°C. Aliquot to avoid freeze/thaw cycles.