Western blot analysis of extracts from K562 cells and Jurkat cells all treated with insulin (0.01U/ml, 15mins), using CaMK1-a (Phospho-Thr177) antibody.
CAMK1 / CAMKI Antibody (phospho-Thr177)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C822199-100
Catalog Number: LS-C822199
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- Description
- CAMKI antibody LS-C822199 is an unconjugated rabbit polyclonal antibody to CAMKI (CAMK1) (pThr177) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB.
- Application
- ELISA, IF, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CaM kinase I | CaM-KI | CAMK1 | CAMKI | CaM kinase I alpha | CaMKI-alpha
- Recommended Dilution: ELISA
- 1:5000
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IHC
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- Q14012
- NCBI GenBank
- NM_003656 | NP_003647.1
- SwissProt
- Q14012
Target
- Target
- Human CAMK1 / CAMKI
- Antigen Species
- Human
- Epitope
- CaMK1-Alpha (Phospho-Thr177) antibody detects endogenous levels of CaMK1-Alpha only when phosphorylated at threonine 177. (Human: Thr177; Mouse: Thr177; Rat: Thr177)
- Gene Name
- CAMK1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Synthesized phosphopeptide derived from human CaMK1-Alpha around the phosphorylation site of threonine 177 (L-S-TP-A-C).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl