LSBio
Calprotectin Antibody (clone 27E10, Biotin)

Calprotectin Antibody (clone 27E10, Biotin)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin
Reactivity:Human
Format:Lyophilized
See all Human Calprotectin antibodies →
$847.00each
Catalog Number: LS-C96224-100

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Overview

Description
Calprotectin antibody LS-C96224 is a biotin-conjugated mouse monoclonal antibody to human Calprotectin. Validated for ELISA and IHC.
Application
ELISA, IHC, IHC-P
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies

Target

Target
Human Calprotectin
Antigen Species
Human
Epitope
The antigen is MRP8/14 (calprotectin), the epitope involves parts of both subunits MRP8 and MRP14. Antigen distribution: Isolated cells: Monocytes carry the antigen both on the surface and intracellularly, granulocytes exhibit it only intracellularly. Up to 80% of monocytes in early cultures (24- 48h) are positive. No reaction has been seen with lymphocytes or platelets. Tissue sections: The antigen is found in macrophages in the red pulp of the spleen and liver. It is strongly expressed in macrophages from acute inflamed tissues (peridontitis, contact eczema, urticaria, erythrodermia) where some endothelial and epidermal cells may also express this antigen. It is normally absent on resident mononuclear phagocytes in healthy tissues (skin, gut, thymus). Specificity: Human: subpopulation of macrophages, monocytes and granulocytes; peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. Other: subpopulation of macrophages of rhesus monkey and bovine tissues. The antibody does not react with swine tissues.

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
27E10

Immunogen

Immunogen
Cultured human monocytes
Immunogen Type
Cell-Based Immunization

Format

Conjugate/Label
Biotin
Format
Lyophilized
Formulation
Lyophilized from PBS pH 7.2, 5mg/ml BSA, and 0.05% Kathon CG
Preservative
No
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C.
Recommended Storage Buffer
PBS

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.