Immunofluorescence staining of CALD1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CALD1 polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoskeleton.
CALD1 / Caldesmon Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C814741-100
Catalog Number: LS-C814741
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- Description
- Caldesmon antibody LS-C814741 is an unconjugated rabbit polyclonal antibody to human Caldesmon (CALD1). Validated for ICC, IF and IHC.
- Application
- ICC, IF, IHC-P
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CALD1 | Caldesmon | Caldesmon 1 | HCAD | LCAD | H-CAD | NAG22 | CDM | L-CAD
- Recommended Dilution: IF/ICC
- 1:300 - 1:10000
- Recommended Dilution: IHC-P
- 1:500 - 1:2000
- UniProt Number
- Q05682
- NCBI GenBank
- NM_004342 | NP_004333.1
- SwissProt
- Q05682
Target
- Target
- Human CALD1 / Caldesmon
- Antigen Species
- Human
- Epitope
- Human CALD1/caldesmon
- Gene Name
- CALD1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to the center region of the Human CALD1/caldesmon
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS