ELISA results of purified Rabbit Anti-CARBONIC ANHYDRASE II Biotin Conjugated Antibody tested against Carbonic Anhydrase II. Each well was coated in duplicate with 1.0 µg of Carbonic Anhydrase II. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-Streptavidin HRP conjugate, and TMB-1000 substrate.
CA2 / Carbonic Anhydrase II Antibody (Lyophilized, Biotin)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Bovine |
| Format: | Lyophilized |
$564.00each
Catalog Number: LS-C209667-100
Catalog Number: LS-C209667
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- Carbonic Anhydrase II antibody LS-C209667 is a biotin-conjugated rabbit polyclonal antibody to bovine Carbonic Anhydrase II (CA2). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Bov
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CA-II | CAII | CAC | Car2 | Carbonate dehydratase II | Carbonic anhydrase C | Carbonic anhydrase II | Carbonic dehydratase | CA2 | Carbonic anhydrase 2 | Carbonic anhydrase B
- Recommended Dilution: ELISA
- 1:2000 - 1:10000
- Recommended Dilution: WB
- 1:200 - 1:1000
- UniProt Number
- P00918
- NCBI GenBank
- NM_000067 | NP_000058.1
- SwissProt
- P00918
Target
- Target
- Bovine CA2 / Carbonic Anhydrase II
- Antigen Species
- Bovine
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes]. Cross reactivity against Carbonic Anhydrase II from other sources may occur but has not been specifically determined.
- Gene Name
- CA2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Carbonic Anhydrase II [Bovine Erythrocytes].
Format
- Conjugate/Label
- Biotin
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl