Western blot analysis of extracts of HL-60 cells, using KIT antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
c-Kit / CD117 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C408201-20
Catalog Number: LS-C408201
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- CD117 antibody LS-C408201 is an unconjugated rabbit polyclonal antibody to human CD117 (c-Kit). Validated for IF, IHC and WB.
- Application
- IF, IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- C-Kit | CD117 | KIT | PBT | Piebald trait | Piebald trait protein | Proto-oncogene c-Kit | SCFR | Soluble KIT variant 1 | Tyrosine-protein kinase Kit | p145 c-kit | CD117 antigen
- Recommended Dilution: IF/ICC
- 1:10 - 1:100
- Recommended Dilution: IHC
- 1:10 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P10721
- NCBI GenBank
- NM_000222 | NP_000213.1
- SwissProt
- P10721
Target
- Target
- Human c-Kit / CD117
- Antigen Species
- Human
- Epitope
- Human C-Kit / CD117
- Gene Name
- KIT
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant fusion protein containing a sequence corresponding to amino acids 25-295 of human KIT (NP_000213.1). SQPSVSPGEPSPPSIHPGKSDLIVRVGDEIRLLCTDPGFVKWTFEILDETNENKQNEWITEKAEATNTGKYTCTNKHGLSNSIYVFVRDPAKLFLVDRSLYGKEDNDTLVRCPLTDPEVTNYSLKGCQGKPLPKDLRFIPDPKAGIMIKSVKRAYHRLCLHCSVDQEGKSVLSEKFILKVRPAFKAVPVVSVSKASYLLREGEEFTVTCTIKDVSSSVYSTWKRENSQTKLQEKYNSWHHGDFNYERQATLTISSARVNDSGVFMCYANNT
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS