Immunohistochemical staining of paraffin-embedded human gastric carcinoma using anti-KIT mouse monoclonal antibody. HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer. (pH8.0) at 120°C for 2.5 minutes.was diluted 1:400 and detection was done with HRP secondary and DAB chromogen. Cytoplasmic and mebraneous positive stain seen in very few tumor cells.
c-Kit / CD117 Antibody (aa27-308, clone UMAB216)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunohistochemistry (general) |
| Reactivity: | Human |
| Format: | Liquid |
$503.00each
Catalog Number: LS-C796981-100
Catalog Number: LS-C796981
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- Description
- CD117 antibody LS-C796981 is an unconjugated mouse monoclonal antibody to human CD117 (c-Kit) (aa27-308). Validated for IHC.
- Application
- IHC
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- C-Kit | CD117 | KIT | PBT | Piebald trait | Piebald trait protein | Proto-oncogene c-Kit | SCFR | Soluble KIT variant 1 | Tyrosine-protein kinase Kit | p145 c-kit | CD117 antigen
- Recommended Dilution: IHC
- 1:400
- UniProt Number
- P10721
- NCBI GenBank
- NM_000222 | NP_000213.1
- SwissProt
- P10721
Target
- Target
- Human c-Kit / CD117
- Antigen Species
- Human
- Epitope
- The specificity of this antibody has been validated by testing against a high-density Protein Microarray containing more than 17,000 recombinant proteins.
- Gene Name
- KIT
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- UMAB216
- Isotype
- IgG2b
- Origin Species
- Human
Immunogen
- Immunogen
- Human recombinant protein fragment corresponding to amino acids 27-308 of human KIT(NP_000213) produced in E.coli.
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS