HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.
BUB1B / BubR1 Antibody (clone OTI6E3)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Western Blot |
| Reactivity: | Dog, Human, Non-Human Primate, Rat |
| Format: | Liquid |
$443.00each
Catalog Number: LS-C114772-100
Catalog Number: LS-C114772
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- BubR1 antibody LS-C114772 is an unconjugated mouse monoclonal antibody to BubR1 (BUB1B) from human. It is reactive with human, rat, dog and other species. Validated for Flow, IF and WB.
- Application
- FC, IF, WB
- Reactivity
- Can, Hu, NHP, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Bub1A | BUBR1 | HBUBR1 | Protein SSK1 | SSK1 | BUB1B | BUB1beta | MAD3L
- Recommended Dilution: FC
- 1:100
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- O60566
- NCBI GenBank
- NM_001211 | NP_001202.4
- SwissProt
- O60566
Target
- Target
- Human BUB1B / BubR1
- Antigen Species
- Human
- Epitope
- Human BUBR1
- Gene Name
- BUB1B
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- OTI6E3
- Isotype
- IgG1
Immunogen
- Immunogen
- Full length human recombinant protein of human BUB1B (NP_001202) produced in HEK293T cell.
- Immunogen Type
- Full-Length Recombinant Protein
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Protein A/G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS