Detection of Mouse and Rat BubR1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
BUB1B / BubR1 Antibody (aa350-400)
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| Antibody: | Rabbit Polyclonal |
| Applications: | Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C287279-10
Catalog Number: LS-C287279
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- Description
- BubR1 antibody LS-C287279 is an unconjugated rabbit polyclonal antibody to BubR1 (BUB1B) (aa350-400) from human. It is reactive with human, mouse and rat. Validated for IP and WB.
- Application
- IP, WB
- Reactivity
- Hu, Ms, Rt
- Predicted Reactivity
- Mml, NHP
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Bub1A | BUBR1 | HBUBR1 | Protein SSK1 | SSK1 | BUB1B | BUB1beta | MAD3L
- Recommended Dilution: WB
- 1:2000 - 1:15000
- UniProt Number
- O60566
- NCBI GenBank
- NM_001211, NP_001202.4
- SwissProt
- O60566
Target
- Target
- Human BUB1B / BubR1
- Antigen Species
- Hu
- Epitope
- Region between residues 350 and 400 of human budding uninhibited by benzimidazoles 1 homolog beta using the numbering given in TrEMBL entry Q8WV50 (GeneID 701).
- Gene Name
- BUB1B
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 701 corresponding to amino acids between 350 and 400 of UniprotID: O60566
- Immunogen Type
- Frag-RP
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- Tris-citrate