Positive WB detected in:K562 whole cell lysate;All lanes: BTRC antibody at 2.8ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 69,66 kDa;Observed band size: 69 kDa;
BTRCP / BETA-TRCP Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$300.00each
Catalog Number: LS-C502254-50
Catalog Number: LS-C502254
For bulk orders or custom formulations:
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- Description
- BETA-TRCP antibody LS-C502254 is an unconjugated rabbit polyclonal antibody to human BETA-TRCP (BTRCP). Validated for ELISA, IF, IHC and WB.
- Application
- ELISA, IF, IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BETA-TRCP | Beta-TrCP1 | BTrCP | BTRC | BetaTrCP | E3RSIkappaB | FBW1A | F-box and WD-repeat protein 1B | FBXW1 | FWD1 | BTrCP1 | FBXW1A
- UniProt Number
- Q9Y297
- NCBI GenBank
- NM_003939 | NP_003930.1
- SwissProt
- Q9Y297
Target
- Target
- Human BTRCP / BETA-TRCP
- Antigen Species
- Human
- Epitope
- Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22'. SCF(BTRC) mediates the ubiquitination of CEP68; this is required for centriole separation during mitosis (PubMed:25704143, PubMed:25503564). SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1/nuclear factor NF-kappa-B p105 subunit, ATF4, CDC25A, DLG1, FBXO5, PER1, SMAD3, SMAD4, SNAI1 and probably NFKB2. Has an essential role in the control of the clock-dependent transcription via degradation of phosphorylated PER1 and PER2. May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase. Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF. Required for proteolytic processing of GLI3.
- Gene Name
- BTRC
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant Human F-box/WD repeat-containing protein 1A protein (397-448AA)
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS