Immunofluorescence staining of BRF1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human BRF1 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.

BRF1 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunocytochemistry, Immunofluorescence
Reactivity:	Human
Format:	Liquid

$309.00each

Catalog Number: LS-C815976-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: BRF1 antibody LS-C815976 is an unconjugated rabbit polyclonal antibody to human BRF1. Validated for ICC and IF.
Application: ICC, IF
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: B-related factor 1 | BRF | B - related factor 1 | BRF1 | HBRF | GTF3B | HTFIIIB90 | TAF3C | TAFIII90 | TFIIIB90 | TAF3B2 | BRF-1 | TF3B90
Recommended Dilution: IF/ICC: 1:100 - 1:500
UniProt Number: Q92994
NCBI GenBank: NM_001519 | NP_001510.2
SwissProt: Q92994

Target

Target: Human BRF1
Antigen Species: Human
Epitope: Human BRF1
Gene Name: BRF1

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: E. coli-derived Human BRF1 fragment
Immunogen Type: Recombinant Protein Fragment

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS, pH 7.0, 0.03% Proclin 300
Volume: 100 Microliter
Preservative: Yes
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Recommended Storage Buffer: PBS