Detection of Human BRD2 by Western Blot. Sample: RIPA extract (10 ug) from HeLa cells that was untreated (-) or treated (+) with CIP (calf intestinal phosphatase). Antibody: Affinity purified goat anti-BRD2 used at 0.05 ug/ml followed by rabbit anti-goat HRP (A50-100P, 1:20000). Detection: Chemiluminescence with 1 minute exposure.
BRD2 / RING3 Antibody (aa550-600)
Primary AntibodyLSBio Guarantee
| Antibody: | Goat Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C287125-10
Catalog Number: LS-C287125
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- Description
- RING3 antibody LS-C287125 is an unconjugated goat polyclonal antibody to human RING3 (BRD2) (aa550-600). Validated for WB.
- Application
- IHC, WB
- Reactivity
- Hu
- Predicted Reactivity
- Bovine
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BRD2 | Bromodomain containing 2 | FSRG1 | KIAA9001 | NaT | RING3 | RNF3 | O27.1.1 | Bromodomain-containing 2 | D6S113E | FSH
- Recommended Dilution: WB
- 1:1000 - 1:10000
- UniProt Number
- P25440
- NCBI GenBank
- NM_005104 | NP_005095.1
- SwissProt
- P25440
Target
- Target
- Human BRD2 / RING3
- Antigen Species
- Human
- Epitope
- Region between residues 550 and 600 of human Bromodomain Containing 2 using the numbering given in entry NP_005095.1 (GeneID 6046).
- Gene Name
- BRD2
Antibody
- Host Species
- Goat
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 6046 corresponding to amino acids between 550 and 600 of UniprotID: P25440
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- Tris-citrate