Immunofluorescence staining of BRE in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human BRE polyclonal antibody (dilution ratio 1:500) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.
BRCC45 / BRE Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C815239-100
Catalog Number: LS-C815239
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- Description
- BRE antibody LS-C815239 is an unconjugated rabbit polyclonal antibody to BRE (BRCC45) from human. It is reactive with human, mouse and rat. Validated for ICC, IF and IHC.
- Application
- ICC, IF, IHC-P
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BRE | BRCA1-A complex subunit BRE | BRCC45 | BRCC4
- Recommended Dilution: IF/ICC
- 1:200 - 1:1000|1:200 - 1:000
- Recommended Dilution: IHC-P
- 1:500 - 1:2000
- UniProt Number
- Q9NXR7
- NCBI GenBank
- NM_004899 | NP_004890.2
- SwissProt
- Q9NXR7
Target
- Target
- Human BRCC45 / BRE
- Antigen Species
- Human
- Epitope
- Human BRE/BRCC45
- Gene Name
- BABAM2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to the C-Terminus of Human BRCC45
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS