Western blot of BRAF (arrow) using rabbit polyclonal BRAF Antibody (S445) (RB10940). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (Lane 2) (Origene Technologies).
BRAF / B-Raf Antibody (Ser445)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C97190-400
Catalog Number: LS-C97190
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- Description
- B-Raf antibody LS-C97190 is an unconjugated rabbit polyclonal antibody to human B-Raf (BRAF) (Ser445). Validated for Flow, IF, IHC and WB.
- Application
- FC, IF, IHC, IHC-P, WB
- Reactivity
- Hu
- Predicted Reactivity
- Chicken, Mouse
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 94 kDa B-raf protein | B-raf | BRAF1 | B Raf | BRAF | Proto-oncogene B-Raf | p94 | B-RAF1 | NS7 | ONCOGENE BRAF | RAFB1
- Recommended Dilution: FC
- 1:10 - 1:50
- Recommended Dilution: IF/ICC
- 1:10 - 1:50
- Recommended Dilution: IHC-P
- 1:10 - 1:50
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P15056
- NCBI GenBank
- NM_004333 | NP_004324.2
- SwissProt
- P15056
Target
- Target
- Human BRAF / B-Raf
- Antigen Species
- Human
- Epitope
- This B-RAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 424-453 amino acids from human B-RAF.
- Gene Name
- BRAF
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Volume
- 400 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS