Western blot analysis of lysates from K562 cells treated with EGF 200ng/ml 30', using B-Raf (Phospho-Thr753) Antibody. The lane on the right is blocked with the phospho peptide.
BRAF / B-Raf Antibody (phospho-Thr753)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$341.00each
Catalog Number: LS-C198697-50
Catalog Number: LS-C198697
For bulk orders or custom formulations:
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- Description
- B-Raf antibody LS-C198697 is an unconjugated rabbit polyclonal antibody to B-Raf (BRAF) (pThr753) from human. It is reactive with human, mouse and rat. Validated for Peptide-ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 94 kDa B-raf protein | B-raf | BRAF1 | B Raf | BRAF | Proto-oncogene B-Raf | p94 | B-RAF1 | NS7 | ONCOGENE BRAF | RAFB1
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P15056
- NCBI GenBank
- NM_004333 | NP_004324.2
- SwissProt
- P15056
Target
- Target
- Human BRAF / B-Raf
- Antigen Species
- Human
- Epitope
- B-Raf (Phospho-Thr753) Antibody detects endogenous levels of B-Raf only when phosphorylated at threonine753.
- Gene Name
- BRAF
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Synthetic peptide derived from human B-Raf around the phosphorylation site of Thr753. (AA Range: 717-766)
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS|150 mM NaCl