Peptide - + Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using B-RAF (Ab-446) antibody.
BRAF / B-Raf Antibody (p446)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C825374-100
Catalog Number: LS-C825374
For bulk orders or custom formulations:
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- Description
- B-Raf antibody LS-C825374 is an unconjugated rabbit polyclonal antibody to B-Raf (BRAF) (p446) from human. It is reactive with human and mouse. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 94 kDa B-raf protein | B-raf | BRAF1 | B Raf | BRAF | Proto-oncogene B-Raf | p94 | B-RAF1 | NS7 | ONCOGENE BRAF | RAFB1
- Recommended Dilution: ELISA
- 1:40000
- Recommended Dilution: IHC
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P15056
- NCBI GenBank
- NM_004333 | NP_004324.2
- SwissProt
- P15056
Target
- Target
- Human BRAF / B-Raf
- Antigen Species
- Human
- Epitope
- B-RAF (Ab-446) Antibody detects endogenous levels of total B-RAF protein.
- Gene Name
- BRAF
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Synthesized non-phosphopeptide derived from human B-RAF around the phosphorylation site of serine 446 (R-D-SP-S-D).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl