All lanes: Anti-BRAF Antibody (C-Term) at 1:2000 dilution Lane 1: Braf-600V -wild type Lane 2: Braf-600E -mutation Lane 3: Raji whole cell lysate Lane 4: Hela whole cell lysate Lane 5: A549 whole cell lysate Lane 6: T47D whole cell lysate Lane 7: MDA-MB-453 whole cell lysate Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
BRAF / B-Raf Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C757537-200
Catalog Number: LS-C757537
For bulk orders or custom formulations:
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- Description
- B-Raf antibody LS-C757537 is an unconjugated rabbit polyclonal antibody to human B-Raf (BRAF). Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC-P, WB
- Reactivity
- Hu
- Predicted Reactivity
- Bovine, Chicken, Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 94 kDa B-raf protein | B-raf | BRAF1 | B Raf | BRAF | Proto-oncogene B-Raf | p94 | B-RAF1 | NS7 | ONCOGENE BRAF | RAFB1
- Recommended Dilution: IHC-P
- 1:25
- Recommended Dilution: WB
- 1:2000
- UniProt Number
- P15056
- NCBI GenBank
- NM_004333 | NP_004324.2
- SwissProt
- P15056
Target
- Target
- Human BRAF / B-Raf
- Antigen Species
- Human
- Gene Name
- BRAF
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 576-606 amino acids from human.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Volume
- 200 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS