Immunofluorescence staining of BMI1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human BMI1 polyclonal antibody (dilution ratio 1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
BMI1 / PCGF4 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C815971-100
Catalog Number: LS-C815971
For bulk orders or custom formulations:
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- Description
- PCGF4 antibody LS-C815971 is an unconjugated rabbit polyclonal antibody to human PCGF4 (BMI1). Validated for ICC and IF.
- Application
- ICC, IF
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BMI1 | BMI-1 | Flvi-2/bmi-1 | FLVI2/BMI1 | PCGF4 | Polycomb group protein Bmi1 | Polycomb complex protein BMI-1 | Dna-binding protein bmi-1 | Polycomb group ring finger 4 | RING finger protein 51 | RNF51
- Recommended Dilution: IF/ICC
- 1:50 - 1:200
- UniProt Number
- P35226
- NCBI GenBank
- NM_005180 | NP_005171.4
- SwissProt
- P35226
Target
- Target
- Human BMI1 / PCGF4
- Antigen Species
- Human
- Epitope
- Human BMI1
- Gene Name
- BMI1|COMMD3-BMI1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- E. coli-derived Human BMI1 fragment
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.0, 0.03% Proclin 300
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS