Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human cervical epithelial adenocarcinoma cell line) cells labeling BMI1 with antibody at 1:25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1:400 dilution (green). Confocal image showing nuclear staining on HeLa cell line. Cytoplasmic actin is detected with Alexa Fluor 555 conjugated with Phalloidin (OB16636430) at 1:100 dilution (red).
BMI1 / PCGF4 Antibody (aa281-314)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C392812-200
Catalog Number: LS-C392812
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- Description
- PCGF4 antibody LS-C392812 is an unconjugated rabbit polyclonal antibody to PCGF4 (BMI1) (aa281-314) from human. It is reactive with human and mouse. Validated for IF, Peptide-ELISA and WB.
- Application
- ELISA, IF, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BMI1 | BMI-1 | Flvi-2/bmi-1 | FLVI2/BMI1 | PCGF4 | Polycomb group protein Bmi1 | Polycomb complex protein BMI-1 | Dna-binding protein bmi-1 | Polycomb group ring finger 4 | RING finger protein 51 | RNF51
- Recommended Dilution: IF/ICC
- 1:25
- Recommended Dilution: WB
- 1:1000 - 1:2000
- UniProt Number
- P35226
- NCBI GenBank
- NM_005180 | NP_005171.4
- SwissProt
- P35226
Target
- Target
- Human BMI1 / PCGF4
- Antigen Species
- Human
- Epitope
- This BMI1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 281-314 amino acids from the C-terminal region of human BMI1.
- Gene Name
- BMI1|COMMD3-BMI1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Volume
- 200 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS