Western blot of full-length, recombinant human Livin using Polyclonal Antibody to Livin/BIRC7/KIAP at 1:2000.
BIRC7 / Livin Antibody (aa199-217)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse, Non-Human Primate |
| Format: | Liquid |
$394.00each
Catalog Number: LS-C148274-50
Catalog Number: LS-C148274
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- Description
- Livin antibody LS-C148274 is an unconjugated rabbit polyclonal antibody to Livin (BIRC7) (aa199-217) from human. It is reactive with human, mouse and monkey. Validated for IHC, IP and WB.
- Application
- IHC, IHC-F, IHC-P, IP, WB
- Reactivity
- Hu, Ms, NHP
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BIRC7 | KIAP | Livin inhibitor of apoptosis | MLIAP | ML-IAP | RNF50 | RING finger protein 50 | LIVIN
- Recommended Dilution: IHC-P
- 1:1000 - 1:5000
- Recommended Dilution: IP
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:1000 - 1:2000
- UniProt Number
- Q96CA5
- NCBI GenBank
- NM_022161 | NP_071444.1
- SwissProt
- Q96CA5
Target
- Target
- Human BIRC7 / Livin
- Antigen Species
- Human
- Epitope
- Livin is a short-lived protein that is thought to be degraded by the ubiquitin-proteasome pathway. Thus users may want to do a time course when examining the effects of treatments on Livin expression. Users are encouraged to refer to the literature for additional information; for example, Ma et al. 2006. 2. Livin has been shown to undergo proteolytic cleavage during apoptosis. Thus the molecular weight of Livin observed on Western blots may vary depending on the presence of absence of cleavage fragments.
- Gene Name
- BIRC7
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- A synthetic peptide corresponding to amino acids 199-217 (PELPTPRREVQSESAQEPG) of human Livin, isoform alpha and isoform beta, GenBank nos. NP_647478 (Livin, isoform alpha) and NP_071444.1 (Livin, isoform beta). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%); Orangutan (95%); Marmoset (84%); Rat (68%).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Antiserum, 0.05% sodium azide
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Unpurified (Serum / Ascites / Supernatant)
Storage & Handling
- Storage Conditions
- Store at -20°C. Aliquot to avoid freeze/thaw cycles.