Immunofluorescence staining of BIRC5 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti- BIRC5 polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.
BIRC5 / Survivin Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C818316-100
Catalog Number: LS-C818316
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- Description
- Survivin antibody LS-C818316 is an unconjugated rabbit polyclonal antibody to human Survivin (BIRC5). Validated for ICC, IF and IHC.
- Application
- ICC, IF, IHC-P
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- API4 | Apoptosis inhibitor survivin | Apoptosis inhibitor 4 | BIRC5 | Survivin | Survivin variant 3 alpha | EPR-1 | IAP4
- Recommended Dilution: IF/ICC
- 1:300 - 1:10000
- Recommended Dilution: IHC-P
- 1:200 - 1:800
- UniProt Number
- O15392
- NCBI GenBank
- NM_001168 | NP_001159.2
- SwissProt
- O15392
Target
- Target
- Human BIRC5 / Survivin
- Antigen Species
- Human
- Epitope
- Human Survivin
- Gene Name
- BIRC5
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Human Survivin / BIRC5 / API4 Protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS