Western blot analysis of extracts from HT-29 cells, treated with EGF (100µg/ml, 30mins), using Survivin (Phospho-Thr34) antibody.
BIRC5 / Survivin Antibody (phospho-Thr34)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C821938-100
Catalog Number: LS-C821938
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- Description
- Survivin antibody LS-C821938 is an unconjugated rabbit polyclonal antibody to Survivin (BIRC5) (pThr34) from human. It is reactive with human and mouse. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- API4 | Apoptosis inhibitor survivin | Apoptosis inhibitor 4 | BIRC5 | Survivin | Survivin variant 3 alpha | EPR-1 | IAP4
- Recommended Dilution: ELISA
- 1:1000
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- O15392
- NCBI GenBank
- NM_001168 | NP_001159.2
- SwissProt
- O15392
Target
- Target
- Human BIRC5 / Survivin
- Antigen Species
- Human
- Epitope
- Survivin (Phospho-Thr34) antibody detects endogenous levels of Survivin only when phosphorylated at threonine 34. (Human: Thr34; Mouse: Thr34)
- Gene Name
- BIRC5
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Synthesized phosphopeptide derived from human Survivin around the phosphorylation site of threonine 34 (A-C-TP-P-E).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl