LSBio
Biotin Antibody (HRP)

ELISA results of purified Goat anti-Biotin Antibody Peroxidase Conjugated tested against purified biotin. Each well was coated in duplicate with 1.0 µg of Bovine Albumin Biotin Conjugated The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.

Biotin Antibody (HRP)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Other, Western Blot
Format:Lyophilized
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$764.00each
Catalog Number: LS-C154189-1

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Overview

Description
Biotin antibody LS-C154189 is an HRP-conjugated goat polyclonal antibody to Biotin. Validated for DB, ELISA, EM, IHC and WB.
Application
DB, ELISA, IHC, Other, WB
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:140000
Recommended Dilution: IHC
1:1000 - 1:5000
Recommended Dilution: WB
1:3000 - 1:15000

Target

Target
Biotin
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, biotinylated bovine serum albumin and Biotinylated IgG.

Antibody

Host Species
Gt
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Biotin conjugated to Keyhole Limpet Hemocyanin (KLH).

Format

Conjugate/Label
HRP
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.