Western Blot of Rabbit anti-Biotin Fluorescein Conjugated Antibody. Lane 1: Biotin Conjugated Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein rabbit secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Guinea Pig IgG. Other band(s): None. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Biotin Antibody (FITC)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunofluorescence, Western Blot
Format:	Lyophilized

$513.00each

Catalog Number: LS-C154264-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: Biotin antibody LS-C154264 is an FITC-conjugated rabbit polyclonal antibody to Biotin. Validated for IF and WB.
Application: IF, WB
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: IF/ICC: 1:500 - 1:2500

Target

Target: Biotin
Epitope: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Biotinylated IgG and Biotinylated Bovine Serum Albumin.

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH).

Format

Conjugate/Label: Fluorescein Isothiocyanate
Format: Lyophilized
Formulation: Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Volume: 100 Microliter
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl