Immunofluorescence Microscopy of Mouse Anti-Biotin antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Biotinylated anti-transferrin receptor antibody. Secondary antibody: CF®488A anti-biotin secondary antibody at 2ug/mL for 45 min at RT. Localization/Staining: plasma membrane and endosome.

Biotin Antibody (clone 4C7.D5)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot
Format:	Liquid

$408.00each

Catalog Number: LS-C153773-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: Biotin antibody LS-C153773 is an unconjugated mouse monoclonal antibody to Biotin. Validated for ELISA, IF and WB.
Application: ELISA, IF, WB
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: ELISA: 1:20000
Recommended Dilution: WB: 1:2000 - 1:10000

Target

Target: Biotin
Epitope: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: 4C7.D5
Isotype: IgG2a
Light Chain: Lambda Light Chain

Immunogen

Immunogen: Anti-Biotin Antibody was produced by repeated immunizations with Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH).

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Preservative: Yes
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl