LSBio
BIN3 Antibody (clone 3A4)

Western blot of Mouse Anti-BIN3 antibody. Lane 1: MEF cells wild type. Lane 2: MEF cells null mice. Load: 35 ug per lane. Primary antibody: BIN3 antibody overnight at 4C. Secondary antibody: IRDye800 mouse secondary antibody for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 29.7 kDa, 30 kDa for BIN-3. Other band(s): non-specific.

BIN3 Antibody (clone 3A4)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
See all Human BIN3 antibodies →
$586.00each
Catalog Number: LS-C153784-100

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Overview

Description
BIN3 antibody LS-C153784 is an unconjugated mouse monoclonal antibody to BIN3 from human. It is reactive with human and mouse. Validated for ELISA, IP and WB.
Application
ELISA, IHC, IP, WB
Reactivity
Hu
Predicted Reactivity
Mouse
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
BIN3 | Bridging integrator 3
Recommended Dilution: ELISA
1:5000 - 1:50000
Recommended Dilution: WB
1:500 - 1:1500
UniProt Number
Q9NQY0
SwissProt
Q9NQY0

Target

Target
Human BIN3
Antigen Species
Human
Epitope
A BLAST analysis was used to suggest cross-reactivity with BIN3 from human and mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with BIN3 from other sources has not been determined.
Gene Name
BIN3

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
3A4
Isotype
IgG1

Immunogen

Immunogen
Anti-BIN3 Monoclonal Antibody was produced in mouse by repeated immunizations with a full length human BIN3 recombinant protein followed by hybridoma development.
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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