Western blot of Bid in normal mouse tissues using Polyclonal Antibody to Bid at 1:2000. BID = recombinant Bid. Arrowheads indicate the positions of the full-length (uncleaved) ~22 kD Bid and the ~15 kD truncated form of Bid (tBid) typical of the caspase-cleavage. Additional bands representing partial Bid degradation products are indicated by asterisks (*).

BID Antibody

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Antibody:	Rabbit Polyclonal
Applications:	Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:	Dog, Human, Mammal (Other), Mouse, Rat
Format:	Liquid

$394.00each

Catalog Number: LS-C148235-50

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Overview

Description: BID antibody LS-C148235 is an unconjugated rabbit polyclonal antibody to BID from mouse. It is reactive with human, mouse, rat and other species. Validated for IHC, IP and WB.
Application: IHC, IHC-F, IHC-P, IP, WB
Reactivity: Can, Hu, Mml, Ms, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: Apoptic death agonist | BID isoform L(2) | BID isoform Si6 | Bh3 | FP497 | p22 BID | BID | BID isoform ES(1b) | Desmocollin type 4 | Human BID coding sequence
Recommended Dilution: IHC-P: 1:1000 - 1:5000
Recommended Dilution: IP: 1:50 - 1:200
Recommended Dilution: WB: 1:1000 - 1:2000
UniProt Number: P55957
NCBI GenBank: NM_001196 | NP_001187.1
SwissProt: P55957

Target

Target: Mouse BID
Antigen Species: Mouse
Epitope: Full-length Bid is known to undergo cleavage/truncation (reviewed in Yin, 2006). Bid was initially found to be cleaved and activated by caspase-8 following death receptor activation. The term "Bid" was first used to described the caspase-8 cleaved/truncated C-terminal Bid. Bid can be also cleaved by other proteases such as Granzyme B, calpains and cathepsin. The C-terminal portion is considered to be the active Bid moiety. This active form can translocate from the cytosol to the mitochondria. Therefore, the appearance of Bid in the mitochondria is considered to be an indication of active Bid. However, it should also be noted that Bid has been shown to translocate to the mitochondria without cleavage in some model systems. The proteolytic cleavage of Bid usually occurs in the unstructured loop region between the alpha 2 and alpha 3 helices, which is between amino acids (aa) 41 and 79 of Bid. For example, the caspase-8/3 cleavage site is at 60 (human) and 59 (mouse). It should be noted that after cleavage, the smaller N-terminal portion of Bid is not necessarily separated from the larger C-terminal portion. The exact size of the C-terminal cleavage products depends on the Bid cleavage sites; generally 11-15 kD C-truncated cleavage forms are generated. Shorter C-terminal Bid cleavage products may result from protease cleavage that involves both caspases and other proteases. Full length Bid is quite stable, but cleaved/truncated forms may have a short half-life (less than 1.5 h) and degrade rapidly. Therefore, a time course may be useful when detecting Bid cleavage fragments.
Gene Name: BID