Flow Cytometry analysis of K562 cells using anti-Human Bid antibody. Overlay histogram showing K562 cells stained with anti-Human Bid antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human Bid Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.