Human Apolipoprotein H Antibody (IgG) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67372-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
BID was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg RAW264.7 Whole Cell Lysate. 2 uL anti-BID rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-BID rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 22 kDa. Observed band size: 22 kDa.
BID Antibody (clone 002, Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C818817-100
Catalog Number: LS-C818817
For bulk orders or custom formulations:
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- Description
- BID antibody LS-C818817 is an unconjugated rabbit monoclonal antibody to human BID. Validated for ELISA, IP and WB.
- Application
- ELISA, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Apoptic death agonist | BID isoform L(2) | BID isoform Si6 | Bh3 | FP497 | p22 BID | BID | BID isoform ES(1b) | Desmocollin type 4 | Human BID coding sequence
- Recommended Dilution: ELISA
- 1:5000 - 1:10000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P55957
- NCBI GenBank
- NM_001196 | NP_001187.1
- SwissProt
- P55957
Target
- Target
- Human BID
- Antigen Species
- Human
- Epitope
- Human BID. Has cross-reactivity in ELISA with Mouse BID
- Gene Name
- BID
Antibody
- Host Species
- Rabbit
- Clonality
- Monoclonal
- Clone
- 002
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Human BID Protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS
