Western blot analysis of Rat Liver showing detection of ~51kDa Beclin 1 protein using Rabbit Anti-Beclin 1 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Rat Liver (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~51kDa.
BECN1 / Beclin-1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Western Blot |
| Reactivity: | Human, Rat |
| Format: | Liquid |
$451.00each
Catalog Number: LS-C775384-100
Catalog Number: LS-C775384
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- Description
- Beclin-1 antibody LS-C775384 is an unconjugated rabbit polyclonal antibody to Beclin-1 (BECN1) from human. It is reactive with human and rat. Validated for IF and WB.
- Application
- IF, WB
- Reactivity
- Hu, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ATG6 | Beclin 1 | Beclin 1, autophagy related | Beclin1 | GT197 | Protein GT197 | VPS30 | Beclin-1 | BECN1
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q14457
- NCBI GenBank
- NM_003766 | NP_003757.1
- SwissProt
- Q14457
Target
- Target
- Human BECN1 / Beclin-1
- Antigen Species
- Human
- Epitope
- Predicted molecular weight at ~51 kDa. Observed a secondary band on some lysates at ~30 kDa.
- Gene Name
- BECN1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide from the mid-protein of human Beclin 1
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS