HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BECN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BECN1.
BECN1 / Beclin-1 Antibody (clone OTI1F1)
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Dog, Human, Rat |
| Format: | Liquid |
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- Description
- Beclin-1 antibody LS-C172820 is an unconjugated mouse monoclonal antibody to Beclin-1 (BECN1) from human. It is reactive with human, rat and dog. Validated for Flow, IF, IHC and WB. Cited in 2 publications.
- Application
- FC, IF, IHC, IHC-P, WB
- Reactivity
- Can, Hu, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- ATG6 | Beclin 1 | Beclin 1, autophagy related | Beclin1 | GT197 | Protein GT197 | VPS30 | Beclin-1 | BECN1
- Recommended Dilution: FC
- 1:100
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: IHC-P
- 1:150
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q14457
- NCBI GenBank
- NM_003766 | NP_003757.1
- SwissProt
- Q14457
Target
- Target
- Human BECN1 / Beclin-1
- Antigen Species
- Human
- Epitope
- Human Beclin 1
- Gene Name
- BECN1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- OTI1F1
- Isotype
- IgG2a
Immunogen
- Immunogen
- Full length human recombinant protein of human BECN1 (NP_003757) produced in HEK293T cell.
- Immunogen Type
- Full-Length Recombinant Protein
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Protein A/G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
The autophagy inhibitor spautin-1, either alone or combined with doxorubicin, decreases cell survival and colony formation in canine appendicular osteosarcoma cells. Courtney R Schott, Latasha Ludwig, Anthony J Mutsaers, Robert A Foster, Geoffrey A Wood. PloS one. 2018 Oct;13:e0206427.
PubMed: 30372478 · PMC: PMC6205606
Beclin-1 is a novel predictive biomarker for canine cutaneous and subcutaneous mast cell tumors. Britta J Knight, Geoffrey A Wood, Robert A Foster, Brenda L Coomber. Veterinary pathology. 2022 Jan;;59:46-56.
PubMed: 34521293 · PMC: PMC8679166