Fluorescent image of U251 cells stained with Beclin 1 antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated Beclin 1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
BECN1 / Beclin-1 Antibody (aa100-123)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C156795-400
Catalog Number: LS-C156795
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- Beclin-1 antibody LS-C156795 is an unconjugated rabbit polyclonal antibody to human Beclin-1 (BECN1) (aa100-123). Validated for IF and WB.
- Application
- IF, WB
- Reactivity
- Hu
- Predicted Reactivity
- Bovine, Chicken, Mouse, Pig, Rat, Xenopus
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ATG6 | Beclin 1 | Beclin 1, autophagy related | Beclin1 | GT197 | Protein GT197 | VPS30 | Beclin-1 | BECN1
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q14457
- NCBI GenBank
- NM_003766 | NP_003757.1
- SwissProt
- Q14457
Target
- Target
- Human BECN1 / Beclin-1
- Antigen Species
- Human
- Epitope
- This Beclin 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-123 amino acids from human Beclin 1.
- Gene Name
- BECN1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Volume
- 400 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS