Human Apolipoprotein H Antibody (IgG) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67372-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Anti-Human BDNF Antibody - Western Blot. Anti-human BDNF antibody in western blot shows detection of recombinant human BDNF raised in E. coli. Recombinant truncated (0.1 ug, 27 kD) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Human BDNF. After washing, membrane was probed with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody ( diluted 1:20000 in blocking buffer (MB-070) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.
BDNF Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Lyophilized |
$579.00each
Catalog Number: LS-C154099-100
Catalog Number: LS-C154099
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- BDNF antibody LS-C154099 is an unconjugated rabbit polyclonal antibody to human BDNF. Validated for ELISA and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ANON2 | Abrineurin | BDNF | BULN2 | Neurotrophin
- Recommended Dilution: ELISA
- 1:10000
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P23560
- NCBI GenBank
- NM_001709 | NP_001700.2
- SwissProt
- P23560
Target
- Target
- Human BDNF
- Antigen Species
- Human
- Epitope
- This antibody is specific for human BDNF protein. A BLAST analysis was used to suggest cross-reactivity with BDNF from human sources based on 100% homology with the immunizing sequence. Based on high to 100% homology, there is a chance of cross-reactivity to BDNF from a wide variety of animals. Cross-reactivity with BDNF from other sources has not been determined.
- Gene Name
- BDNF
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated human BDNF protein produced in E.coli.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
- Concentration
- 1 mg/mL
- Preservative
- No
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
