
Western blot analysis of 100 ng Bcl-2 using purified rabbit anti-Bcl-2 primary antibody (1:5,000 µg/ml). The blot was incubated with anti-rabbit HRP-conjugated IgG (1:10,000).
BCL2 / Bcl-2 Antibody (N-term, clone 13G6.D3, Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Lyophilized |
$247.00each
Catalog Number: LS-C149224-20
Catalog Number: LS-C149224
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- Description
- Bcl-2 antibody LS-C149224 is an unconjugated mouse polyclonal antibody to human Bcl-2 (BCL2) (N-term). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Apoptosis regulator Bcl-2 | BCL2 | B-cell CLL/lymphoma 2 | B-cell lymphoma protein 2 | Bcl-2 | PPP1R50
- Recommended Dilution: ELISA
- 1:40000
- Recommended Dilution: WB
- 1:5000
- UniProt Number
- P10415
- NCBI GenBank
- NM_000633 | NP_000624.2
- SwissProt
- P10415
Target
- Target
- Human BCL2 / Bcl-2
- Antigen Species
- Human
- Epitope
- This antibody was selected for its ability to specifically detect Bcl-2 from humans. There is no cross reactivity with Caspase-3, Caspase-8 or Livin.
- Gene Name
- BCL2
Antibody
- Host Species
- Mouse
- Clonality
- Polyclonal
- Clone
- 13G6.D3
- Isotype
- IgG2a
Immunogen
- Immunogen
- KLH-conjugated synthetic peptide of Bcl-2 (N-term). Immunogen Sequence: CMKYIHYKLSQRGYEWDAGD.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized from PBS, No preservatives added
- Preservative
- No
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Short term: Store at 4°C for up to 1 month. Long term: Store at -20°C to -80°C for up to 18 months. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
