Formalin-fixed and paraffin-embedded human testis tissue reacted with Bcl-2 BH3 Domain Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
BCL2 / Bcl-2 Antibody (aa75-110)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human, Rat |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C98842-400
Catalog Number: LS-C98842
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- Description
- Bcl-2 antibody LS-C98842 is an unconjugated rabbit polyclonal antibody to Bcl-2 (BCL2) (aa75-110) from human. It is reactive with human and rat. Validated for ICC, IF, IHC and WB. Cited in 1 publication.
- Application
- ICC, IF, IHC, IHC-P, WB
- Reactivity
- Hu, Rt
- Predicted Reactivity
- Bovine
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Apoptosis regulator Bcl-2 | BCL2 | B-cell CLL/lymphoma 2 | B-cell lymphoma protein 2 | Bcl-2 | PPP1R50
- Recommended Dilution: IF/ICC
- 1:10 - 1:50
- Recommended Dilution: IHC-P
- 1:10 - 1:50
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P10415
- NCBI GenBank
- NM_000633 | NP_000624.2
- SwissProt
- P10415
Target
- Target
- Human BCL2 / Bcl-2
- Antigen Species
- Human
- Epitope
- This Bcl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-110 amino acids from human Bcl.
- Gene Name
- BCL2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Volume
- 400 Microliter
- Preservative
- Yes
- Purification Method
- Ammonium Sulfate Precipitation
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS
Natural diterpenoid compound elevates expression of Bim protein, which interacts with antiapoptotic protein Bcl-2, converting it to proapoptotic Bax-like molecule. Zhao L, He F, Liu H, Zhu Y, Tian W, Gao P, He H, Yue W, Lei X, Ni B, Wang X, Jin H, Hao X, Lin J, Chen Q. The Journal of biological chemistry. 2012;287:1054-65. PubMed: 22065578 PMC: PMC3256916