Western blot analysis of extracts of mouse pancreas, using BCKDHB antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.

BCKDHB Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Western Blot
Reactivity:	Mouse
Format:	Liquid

$281.00each

Catalog Number: LS-C497242-20

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Overview

Description: BCKDHB antibody LS-C497242 is an unconjugated rabbit polyclonal antibody to mouse BCKDHB. Validated for WB.
Application: WB
Reactivity: Ms
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: BCKDH E1-beta | BCKDHB | DJ279A18.1 | E1B | BCKDE1B
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: P21953
NCBI GenBank: NM_000056 | NP_000047.1
SwissProt: P21953

Target

Target: Human BCKDHB
Antigen Species: Human
Gene Name: BCKDHB

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 183-392 of human BCKDHB (NP_898871.1). RSPWGCVGHGALYHSQSPEAFFAHCPGIKVVIPRSPFQAKGLLLSCIEDKNPCIFFEPKILYRAAAEEVPIEPYNIPLSQAEVIQEGSDVTLVAWGTQVHVIREVASMAKEKLGVSCEVIDLRTIIPWDVDTICKSVIKTGRLLISHEAPLTGGFASEISSTVQEECFLNLEAPISRVCGYDTPFPHIFEPFYIPDKWKCYDALRKMINY
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)