Western blot analysis of extracts from HepG2 cells treated with EGF (200ng/ml, 30mins) and A549 cells treated with PMA (125ng/ml, 30mins), using p130 Cas (Phospho-Tyr249) antibody.
BCAR1 / p130Cas Antibody (phospho-Tyr249)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C822035-100
Catalog Number: LS-C822035
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- Description
- P130Cas antibody LS-C822035 is an unconjugated rabbit polyclonal antibody to p130Cas (BCAR1) (pTyr249) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CAS | CAS1 | CRKAS | BCAR1 | CASS1 | CRK-associated substrate | p130Cas
- Recommended Dilution: ELISA
- 1:10000
- Recommended Dilution: IHC
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P56945
- NCBI GenBank
- NM_014567 | NP_055382.2
- SwissProt
- P56945
Target
- Target
- Human BCAR1 / p130Cas
- Antigen Species
- Human
- Epitope
- p130 Cas (Phospho-Tyr249) antibody detects endogenous levels of p130 Cas only when phosphorylated at tyrosine 249. (Human: Tyr249; Mouse: Tyr253; Rat: Tyr347)
- Gene Name
- BCAR1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Synthesized phosphopeptide derived from human p130 Cas around the phosphorylation site of tyrosine 249 (D-I-YP-D-V).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl