Western blot analysis of extracts of PC-12 cells, using Phospho-BCAR1-Y165 antibody at 1:1000 dilution. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 90s.
BCAR1 / p130Cas Antibody (phospho-Tyr165)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$293.00each
Catalog Number: LS-C750619-20
Catalog Number: LS-C750619
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- Description
- P130Cas antibody LS-C750619 is an unconjugated rabbit polyclonal antibody to p130Cas (BCAR1) (pTyr165) from human. It is reactive with human, mouse and rat. Validated for WB.
- Application
- WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CAS | CAS1 | CRKAS | BCAR1 | CASS1 | CRK-associated substrate | p130Cas
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P56945
- NCBI GenBank
- NM_014567 | NP_055382.2
- SwissProt
- P56945
Target
- Target
- Human BCAR1 / p130Cas
- Antigen Species
- Human
- Gene Name
- BCAR1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS