Western blot of BAR in normal prostate and prostate carcinoma tissue lysates. 25 ug protein was loaded per lane. Tissue lysates from 15 different prostate carcinoma patients show variable expression of BAR with respect to banding patterns and amount of BAR expression. Major BAR bands typically migrate as a single band or as a doublet. These bands are typically observed at ~50-54 kD. This is higher than the predicted molecular weight from the 450 amino acid BAR sequence, and may represent phosphorylation or other post-translational modifications. N = tissue lysate from normal prostate.
BAR / BFAR Antibody (aa3-21)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$394.00each
Catalog Number: LS-C148222-50
Catalog Number: LS-C148222
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- Description
- BFAR antibody LS-C148222 is an unconjugated rabbit polyclonal antibody to human BFAR (BAR) (aa3-21). Validated for IHC, IP and WB.
- Application
- IHC, IHC-F, IHC-P, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BFAR | BAR | RNF47 | RING finger protein 47
- Recommended Dilution: IHC-P
- 1:1000 - 1:5000
- Recommended Dilution: IP
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:1000 - 1:2000
- UniProt Number
- Q9NZS9
- NCBI GenBank
- NM_016561 | NP_057645.1
- SwissProt
- Q9NZS9
Target
- Target
- Human BAR / BFAR
- Antigen Species
- Human
- Epitope
- BAR.
- Gene Name
- BFAR
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- A synthetic peptide corresponding to amino acids 3-21 (EPQKSYVNTMDLERDEPLK) of human BAR, GenBank no. NP_057645.1. Human BAR is a 450 amino acid protein. Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (84%).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Antiserum, 0.05% sodium azide
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Unpurified (Serum / Ascites / Supernatant)
Storage & Handling
- Storage Conditions
- Store at -20°C. Aliquot to avoid freeze/thaw cycles.