LSBio
BAD Antibody

Immunofluorescence microscopy of BAD using Polyclonal Antibody to BAD at 1:2000. Du145 human prostate carcinoma cells were cultured without (A) or with (B and C) 1 uM of the triphosphatase inhibitor thapsigargin (THG) for 12 hr. A and B, staining with BAD antibody, followed by a FITC-conjugated secondary antibody. C, staining with a mitochondrial marker (antibody to mitochondrial Hsp60), followed by a rhodamine-conjugated secondary antibody. THG induces Ca2+ release from internal stores which can promote apoptosis. BAD staining was located diffusely throughout the cytoplasm of untreated cells (A), and localized to the mitochondria in treated cells (B).

BAD Antibody

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
See all Human BAD antibodies →
$394.00each
Catalog Number: LS-C148207-50

For bulk orders or custom formulations:

Request a Quote

Overview

Description
BAD antibody LS-C148207 is an unconjugated rabbit polyclonal antibody to human BAD. Validated for Flow, IHC, IP and WB.
Application
FC, IHC, IHC-F, IHC-P, IP, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
BBC2 | Bcl-2-like protein 8 | BCL2-binding component 6 | BCL2L8 | BBC6 | BCL-X/BCL-2 binding protein | Bcl-2-binding component 6 | BAD | Bcl2 antagonist of cell death | BCL2-binding protein | Bcl2-L-8
Recommended Dilution: IHC-P
1:1000 - 1:5000
Recommended Dilution: IP
1:50 - 1:200
Recommended Dilution: WB
1:1000 - 1:2000
UniProt Number
Q92934
SwissProt
Q92934

Target

Target
Human BAD
Antigen Species
Human
Epitope
Bad.
Gene Name
BAD

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
A full length recombinant protein corresponding to human Bad.
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Antiserum, 0.05% sodium azide
Volume
50 Microliter
Preservative
Yes
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.