Immunohistochemistry of Rabbit anti-Beta-2-Microglobulin Antibody. Tissue: normal human skin. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Left panel: isotype control, Right panel: β2 microglobulin antibody at 1 ug/ml for 20 min at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: β2 microglobulin is cell membrane (and to lesser amount cytoplasmatic compartment). Staining: Beta-2-Microglobulin as brown with diaminobenzidine and with a hematoxylin purple counterstain.
B2M / Beta 2 Microglobulin Antibody (Lyophilized, HRP)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Lyophilized |
$619.00each
Catalog Number: LS-C59573-500
Catalog Number: LS-C59573
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- Description
- Beta 2 Microglobulin antibody LS-C59573 is an HRP-conjugated rabbit polyclonal antibody to human Beta 2 Microglobulin (B2M). Validated for ELISA, IF, IHC and WB.
- Application
- DB, ELISA, IF, IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- B2M | Beta 2 Microglobulin | Beta-2-microglobulin | Beta-2-microglobin
- Recommended Dilution: ELISA
- 1:2000 - 1:10000
- Recommended Dilution: IF/ICC
- 1:200
- Recommended Dilution: IHC
- 1:200 - 1:1000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P61769
- NCBI GenBank
- NM_004048 | NP_004039.1
- SwissProt
- P61769
Target
- Target
- Human B2M / Beta 2 Microglobulin
- Antigen Species
- Human
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum, as well as purified and partially purified b2-Microglobulin (Human Urine). Cross reactivity against b2-Microglobulin from other sources is unknown.
- Gene Name
- B2M
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Anti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with beta-2-Microglobulin protein isolated from human urine.
- Immunogen Type
- Native Protein (Purified from Natural Source)
Format
- Conjugate/Label
- Horseradish Peroxidase
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store -20°C. Avoid freeze-thaw cycles. Protect from light. Store undiluted.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl