Dot Blot results of rabbit Anti-Beta 2 Microglobulin Biotin Conjugated. Antigen: Beta-2-Microglobulin. Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng. Blocking: MB-070 Buffer for 30 minutes at RT. Primary Antibody: Rabbit Anti-Beta-2-Microglobulin Biotin 10µg/mL for 1hr at RT. Secondary Antibody: Streptavidin-HRP at 1:40,000 for 30min at RT. Imaging System ChemiDoc, Filter used: Chemi.
B2M / Beta 2 Microglobulin Antibody (Lyophilized, Biotin)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Lyophilized |
$564.00each
Catalog Number: LS-C745053-100
Catalog Number: LS-C745053
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- Description
- Beta 2 Microglobulin antibody LS-C745053 is a biotin-conjugated rabbit polyclonal antibody to human Beta 2 Microglobulin (B2M). Validated for DB, ELISA, IHC and WB.
- Application
- DB, ELISA, IHC, WB
- Reactivity
- Hu
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- B2M | Beta 2 Microglobulin | Beta-2-microglobulin | Beta-2-microglobin
- Recommended Dilution: ELISA
- 1:2000 - 1:10000
- Recommended Dilution: IHC
- 1:500 - 1:3000
- Recommended Dilution: WB
- 1:1000 - 1:5000
- UniProt Number
- P61769
- NCBI GenBank
- NM_004048, NP_004039.1
- SwissProt
- P61769
Target
- Target
- Human B2M / Beta 2 Microglobulin
- Antigen Species
- Hu
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified b2-Microglobulin [Human Urine]. Cross reactivity against b2-Microglobulin from other sources may occur but has not been specifically determined.
- Gene Name
- B2M
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Hu
Immunogen
- Immunogen
- Anti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with beta-2-Microglobulin protein isolated from human urine.
- Immunogen Type
- Native
Format
- Conjugate/Label
- Biotin
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- IEX
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl