Immunofluorescence staining of B2M in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-human B2M monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm and cell membrane.
B2M / Beta 2 Microglobulin Antibody (clone 34, Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$164.00each
Catalog Number: LS-C819609-20
Catalog Number: LS-C819609
For bulk orders or custom formulations:
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- Description
- Beta 2 Microglobulin antibody LS-C819609 is an unconjugated mouse monoclonal antibody to human Beta 2 Microglobulin (B2M). Validated for ELISA, Flow, ICC, IF, IHC and WB.
- Application
- ELISA, FC, ICC, IF, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- B2M | Beta 2 Microglobulin | Beta-2-microglobulin | Beta-2-microglobin
- Recommended Dilution: ELISA
- 1:1000 - 1:10000
- Recommended Dilution: FC
- 1:25 - 1:100
- Recommended Dilution: IF/ICC
- 1:20 - 1:100
- Recommended Dilution: IHC-P
- 1:30 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P61769
- NCBI GenBank
- NM_004048 | NP_004039.1
- SwissProt
- P61769
Target
- Target
- Human B2M / Beta 2 Microglobulin
- Antigen Species
- Human
- Epitope
- Human Beta-2 microglobulin
- Gene Name
- B2M
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 34
- Isotype
- IgG1
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Human Beta-2 microglobulin/B2M Protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 20 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS